Purification and Characterization of Leech Hyaluronic Acid-endo- P-glucuronidase*
نویسنده
چکیده
In 1960, Linker, Meyer, and Hoffman (1) reported that a crude leech preparation hydrolyzes the glucuronide linkages of hyaluronic acid, unlike bacterial and testes hyaluronidases, which act on glucosaminide linkages. This paper deals with the first systematic attempt to purify the leech P-glucuronidase that specifically hydrolyzes hyaluronic acid. Purified preparations of the enzyme have indeed been prepared, a unit of enzyme activity has been tentatively defined, and a study has been made of a number of properties of the enzyme. This enzyme differs greatly from existing /I-glucuronidases, not only with respect to substrate specificity but also with regard to its sensitivity to inhibitors.
منابع مشابه
PURIFICATION, PAHflAL CHARACTERIZATION OF RAT KIDNEY HYALURONIC ACID-BINDING PROTEIN AND ITS LOCALIZATION ON THE CELL
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